Translational_Unit

Part:BBa_K395160

Designed by: Kaneta Yusuke   Group: iGEM10_Tokyo_Tech   (2010-10-02)

RBS-CmR(no promoter and terminator)

Fig.1 Growth curve of the cell introduced PlacIq-CmR (BBa_K395165,blue curves), Plux-CmR (BBa_K395162,Red curves), or promoterless-CmR (BBa_K395160,green curves) with or without AHL (3OC6HSL).
This work is done by Yusuke Kaneta.
We got this part, RBS-CmR, from BBa_P1004 by PCR.this doesn't have any promoters and terminators.

This part enables to tune threshold of resistance for chloramphenicol, exchanging the promoters.
For more information, see [http://2010.igem.org/Team:Tokyo_Tech/Project/Artificial_Cooperation_System/Cm_assay our work in Tokyo_Tech 2010 wiki]
















Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None